The Regulus I tactical missile was essentially the same as the flight test missile, except the tactical missile had no landing gear and carried a nuclear warhead.  Regulus I missiles could be launched by aircraft carriers and cruisers as well as submarines.  Five submarines  outfitted with Regulus I missiles engaged in deterrent patrols up through 1964.

Regulus:

Proposal Characteristics
ABLE, BAKER, CHARLIE and FTV-1
FTV-2
Operation SPLASH and FTV-3
Regulus I Sub-Systems
Regulus I and II